Coding

Part:BBa_J36843:Design

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)


Streptavidin, single-chain dimer + His6 tag (no start codon)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 787
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 217
    Illegal AgeI site found at 751
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a PstI site CTGCAG at nucleotides 617-622 of the part, which was mutated to CTGCGG.

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.


Source

PCR off a plasmid from Filiz Aslan at Boston University.

References